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Problem 1: (10 pts) write down the functions of the following reagents (words limit for
each question: 30 words).
a. 0.25% Trypsin
c. Hoechst 3342
d. Propidium iodide (PI staining)
e. ALP staining
Problem 2. (20pts) Are the following descriptions correct, if not, correct it.
(words limit for each question: 30 words).
a. DAPI can be used to stain cell nucleus.
b. Hoechst 3342 can be used to stain cell nucleus.
c. Propidium iodide can be used to stain cell nucleus.
d. The cells (cells we used in class, HeLa, MDA-MB-231) can grow on petri dish without
e. In class, we use warmed medium for cell culture.
f. In class, we use normal cell culture medium to freeze cells.